Journal: International Journal of Molecular Sciences

Article Title: Application of hs-CRP in Assessment of Tissue Inflammation Following Implantation of Biodegradable Polymer in Experiment

doi: 10.3390/ijms252011183

Figure Lengend Snippet: Changes in blood hs-CRP levels over time, ELISA (mg/mL).

Article Snippet: Then, the samples were studied using the ELISA kit for highly sensitive C-reactive protein (hs-CRP) (Elabscience, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Translational Psychiatry

Article Title: Decoding the inflammatory signature of the major depressive episode: insights from peripheral immunophenotyping in active and remitted condition, a case–control study

doi: 10.1038/s41398-024-02902-2

Figure Lengend Snippet: A , B Cytokines, chemokines, and neurotrophic factors, were assessed employing a customized LEGENDPlex system for a human inflammatory panel 1 ( A ), and a neuroinflammation panel ( B ) determined by flow cytometry. Each panel allow the simultaneous quantification of all assessed molecules in 50 µL of the sample. The concentrations of each cytokine are determined by a standard curve provided by the kit, see Supplemental Figs. and . C IL-6 was determined by high-sensitivity (HS) ELISA kit. Independent data for each molecule is graphed and the median of each group is shown. Human Inflammatory Panel 1 includes: IL-1β, IFNγ, IL-17A, IL-33, CXCL8, IL-10, IL-12p70 and IL-23, and the Human Neuroinflammation Panel includes: β-NGF, CX3CL1, BDNF, sTREM2, IL-18, TNFα and CCL2. Major Depression Episode (MDE), active vs remitted MDE, Healthy Control (HC). Statistical differences among groups was calculated by Kruskal–Wallis rank sum test with Bonferroni adjustment for multiple comparisons . ** P -value < 0.01; * P -value < 0.05.

Article Snippet: Considering that the IL-6 measurement was under the low range of detection of the legendPlex system, we have measured this cytokine in the plasma of HC and patients with MDE employing a highly sensitive ELISA kit (Enzo Life Sciences, Supplementary Table ).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Avicenna Journal of Medical Biotechnology

Article Title: Effect of Intra-ovarian Injection of Mesenchymal Stem Cells or its Conditioned Media on Repeated OPU-IVEP Outcomes in Jersey Heifers and Its Relationship with Follicular Fluid Inflammatory Markers

doi: 10.18502/ajmb.v16i1.14167

Figure Lengend Snippet: The follicular fluid concentrations of Resolvin E1 (RVE1 pg/ml ) and Interleukin-12 (IL-12 ng/L ) in different OPU sessions (1, 5, 6 and 9) and 4 weeks after session 11 and the total number of blastocysts produced on Day 7 and Day 8 (TotD7D8) in Jersey heifers (n=8) that were subjected to 11 OPU sessions and received intraovarian administration of MSCs or ConM (right ovary) and Dulbecco’s Modified Phosphate Buffer Saline (DMPBS; left ovary) on OPU sessions 5 and 8 and 2 weeks after session 11. Statistical analysis of data in pre-treatment (1 to 5) and treatment (6 to 11) sessions were analyzed separately. Data were presented as mean±SEM. Ab) Values within the group during sessions 1–5 with different letters differ (p<0.05). AB) Values within the group during sessions 6–11 with different letters differ (p<0.05).

Article Snippet: The concentration of RvE1 was assayed by bovine highly sensitive RvE1 ELISA kit (Cat. No: ZB-hs12042C-Bo9648; ZellBio GmbH, Germany), with the assay range of 2.5–80 pg/ml and sensitivity of 0.3 pg/ml .

Techniques: Produced, Modification, Saline

Journal: Avicenna Journal of Medical Biotechnology

Article Title: Effect of Intra-ovarian Injection of Mesenchymal Stem Cells or its Conditioned Media on Repeated OPU-IVEP Outcomes in Jersey Heifers and Its Relationship with Follicular Fluid Inflammatory Markers

doi: 10.18502/ajmb.v16i1.14167

Figure Lengend Snippet: The concentration of Interleukin-12 (IL-12) and Resolvine-E1 (RvE1) in follicular fluid of left and right ovaries, in pre-treatment (Sessions 1–5), and treatment sessions (Sessions 6–11). Data were presented as mean± SEM

Article Snippet: The concentration of RvE1 was assayed by bovine highly sensitive RvE1 ELISA kit (Cat. No: ZB-hs12042C-Bo9648; ZellBio GmbH, Germany), with the assay range of 2.5–80 pg/ml and sensitivity of 0.3 pg/ml .

Techniques: Concentration Assay, Control

Journal: Avicenna Journal of Medical Biotechnology

Article Title: Effect of Intra-ovarian Injection of Mesenchymal Stem Cells or its Conditioned Media on Repeated OPU-IVEP Outcomes in Jersey Heifers and Its Relationship with Follicular Fluid Inflammatory Markers

doi: 10.18502/ajmb.v16i1.14167

Figure Lengend Snippet: Correlation of Resolvine-E1 (RvE1) and Interleukin-12 (IL-12) concentrations in follicular fluid with varying size of follicles, oocyte, and embryo development

Article Snippet: The concentration of RvE1 was assayed by bovine highly sensitive RvE1 ELISA kit (Cat. No: ZB-hs12042C-Bo9648; ZellBio GmbH, Germany), with the assay range of 2.5–80 pg/ml and sensitivity of 0.3 pg/ml .

Techniques: Cell Culture